Composite

Part:BBa_K5317021:Design

Designed by: Vanessa Bruhn   Group: iGEM24_Hannover   (2024-09-22)


CMV-ATF2-mRuby2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1398
    Illegal EcoRI site found at 1660
    Illegal XbaI site found at 1373
    Illegal XbaI site found at 1701
    Illegal PstI site found at 2108
    Illegal PstI site found at 2557
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 670
    Illegal SapI.rc site found at 2408


Design Notes

This composite part was codon optimised for human cell lines.


Source

The fluorescent marker mRuby was obtained from Eurofins and ATF2 was acquired from S. aureus.